anti human anti slug  (St Johns Laboratory)

Journal: iScience

Article Title: Lupeol synergizes with 5-fluorouracil to combat c-MET/EphA2 mediated chemoresistance in triple negative breast cancer

doi: 10.1016/j.isci.2023.108395

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Article Snippet: Mouse monoclonal anti human slug (4B6D5) , Thermo Fisher Scientific , Cat# MA5-38634; RRID: AB_2898546.

Techniques: Recombinant, Modification, Membrane, Transfection, In Vivo, Software

Journal: Cell reports

Article Title: Breast cancer cell mesenchymal transition and metastasis directed by DAP5/eIF3d-mediated selective mRNA translation

doi: 10.1016/j.celrep.2023.112646

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-human/mouse Snail2/Slug antibody , Cell Signaling Technology , Cat# 9585/RRID:AB_2239535.

Techniques: Recombinant, Staining, Protease Inhibitor, Virus, Reverse Transcription, SYBR Green Assay, Bicinchoninic Acid Protein Assay, MTT Assay, Software, Imaging

Journal: Cancers

Article Title: Cancer Cell Biomechanical Properties Accompany Tspan8-Dependent Cutaneous Melanoma Invasion.

doi: 10.3390/cancers16040694

Figure Lengend Snippet: Figure 3. EMT-TFs regulate the expression of Tspan8, crucial for cutaneous melanoma invasion. (a) qPCR analysis of SNAI2, ZEB2, or TSPAN8 transcript expression levels 72 h after SNAI2 (left panel), ZEB2 (right panel), or control (both panels) siRNA transfection in non-invasive (IC8) or invasive (T1C3 and C-09.10) melanoma cells. (b) SNAI2, ZEB2, and Tspan8 expression measured by Western blot analysis 72 h after SNAI2 (left panel), ZEB2 (right panel), or control (both panels) siRNA transfection in T1C3 or C-09.10 melanoma cells. (c) (Top panel), qPCR analysis of TSPAN8 transcript expression level in C-09.10-ZEB1 compared to C-09.10-CT melanoma cells. (Bottom panel), ZEB1 and Tspan8 expression measured by Western blot analysis in C-09.10-CT and C-09.10-ZEB1 melanoma cells. (d) ZEB1 chromatin immunoprecipitation (ChIP) assays performed in C-09.10 melanoma cells, using IgG antibody as a negative control. Enrichment of TSPAN8 promoter region was analyzed by qPCR in comparison with a negative control promoter region located –1 kb upstream of the beginning of pTSPAN8 or a positive control using MITF promoter region. Results are representa- tive of three independent experiments. (e) (Upper left panel), qPCR analysis of SNAI2, ZEB1, or TSPAN8 transcript expression levels in healthy skin, melanoma, or local metastasis conditions of mitf::Xmrk/+ medaka. (Upper right panel), ZEB1 and Tspan8 expression measured by Western blot analysis in healthy skin versus melanoma conditions of mitf::Xmrk/+ medaka. (Lower panel), Tspan8 staining in areas of invasive melanoma by immunohistochemistry on melanoma sections from a mitf::Xmrk/+ medaka fish, using a control antibody (serum before immunization, (left image) or a custom antibody directed against Tspan8 (serum after immunization, (right image). (f) (Left panel), quan- tification of Tspan8 expression level, based on the immunoscore previously established (47), in ZEB1low

Article Snippet: The used antibodies were as follows: mouse monoclonal TS29 clone (1/500) for human Tspan8 detection; sc-166476 mouse antibody (1/500, Santa Cruz, CA, USA) for human SNAI2 detection; rabbit monoclonal antibody HPA027524 (RRID:AB_1844977, Sigma, Burbank, CA, USA) for human ZEB2 detection; rabbit monoclonal antibody HPA003456 (1/500, RRID: AB_10603840, Sigma, USA) for human ZEB1 detection; custom antibody elaborated from rabbit serum before and after immunization (Covalab, Bron, France) for Medaka Tspan8 detection; mouse monoclonal anti-actin clone C4 antibody (1/5000; MAB1501, Millipore, Darmstadt, Germany) for both human and medaka β-actin detection.

Techniques: Expressing, Control, Transfection, Western Blot, Chromatin Immunoprecipitation, Negative Control, Comparison, Positive Control, Staining, Immunohistochemistry

Journal: Cancers

Article Title: Cancer Cell Biomechanical Properties Accompany Tspan8-Dependent Cutaneous Melanoma Invasion.

doi: 10.3390/cancers16040694

Figure Lengend Snippet: Figure 4. Tspan8 modulation regulates stiffness and morphological properties of cutaneous melanoma cells. (a) Global stiffness measurements of different melanoma cell constructs according to their Tspan8 expression levels measured by Western blot analysis (bottom panels). (Top left panel), non- invasive melanoma cells with an empty vector versus melanoma cells with a vector allowing ectopic

Article Snippet: The used antibodies were as follows: mouse monoclonal TS29 clone (1/500) for human Tspan8 detection; sc-166476 mouse antibody (1/500, Santa Cruz, CA, USA) for human SNAI2 detection; rabbit monoclonal antibody HPA027524 (RRID:AB_1844977, Sigma, Burbank, CA, USA) for human ZEB2 detection; rabbit monoclonal antibody HPA003456 (1/500, RRID: AB_10603840, Sigma, USA) for human ZEB1 detection; custom antibody elaborated from rabbit serum before and after immunization (Covalab, Bron, France) for Medaka Tspan8 detection; mouse monoclonal anti-actin clone C4 antibody (1/5000; MAB1501, Millipore, Darmstadt, Germany) for both human and medaka β-actin detection.

Techniques: Construct, Expressing, Western Blot, Plasmid Preparation